Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/89345
48 Visitors
53 Hits
0 Downloads
- Title
- GlycoSpectrumScan : fishing glycopeptides from MS spectra of protease digests of human colostrum sIgA
- Related
- Journal of proteome research, Vol. 9, Issue 2 (2010), p.1063-1075
- DOI
- 10.1021/pr900956x
- Publisher
- American Chemical Society
- Date
- 2010
- Author/Creator
- Deshpande, Nandan
- Author/Creator
- Jensen, Pia H
- Author/Creator
- Packer, Nicolle H
- Author/Creator
- Kolarich, Daniel
- Description
- With the emergence of glycoproteomics, there is a need to develop bioinformatic tools to identify glycopeptides in protease digests of glycoproteins. GlycoSpectrumScan is a web-based tool that identifies the glycoheterogeneity on a peptide from mass spectrometric data. Two experimental data sets are required as inputs: (1) oligosaccharide compositions of the N- and/or O-linked glycans present in the sample and (2) in silico derived peptide masses of proteolytically digested proteins with a potential number of N- and/or O-glycosylation sites. GlycoSpectrumScan uses MS data, rather than MS/MS data, to identify glycopeptides and determine the relative distribution of N- and O-glycoforms at each site. It is functional for assigning monosaccharide compositions on glycopeptides with single and multiple sites of glycosylation. The algorithm allows the input of raw mass data, including multiply charged ions, making it applicable for both ESI and MALDI data from all mass spectrometer platforms. Manual analysis time for identifying glycosylation heterogeneity at each site on glycoprotein(s) is substantially decreased. The application of this tool to characterize the N- and O-linked glycopeptides from human secretory IgA (sIgA), consisting of secretory component (7 N-linked sites), IgA1 (2 N-linked, ≤5 O-linked sites), IgA2 (4 N-linked sites) and J-chain (1 N-linked site) is described. GlycoSpectrumScan is freely available at www.glycospectrumscan.org.
- Description
- 13 page(s)
- Subject Keyword
- bioinformatics
- Subject Keyword
- glycoinformatics
- Subject Keyword
- colostrum
- Subject Keyword
- N-glycan
- Subject Keyword
- O-glycan
- Subject Keyword
- glycosylation
- Subject Keyword
- sIgA
- Subject Keyword
- glycoproteomics
- Subject Keyword
- glycopeptide
- Subject Keyword
- graphitized carbon
- Subject Keyword
- mass spectrometry
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/89345
- Identifier
- ISSN:1535-3907
- Identifier
- mq-rm-2009008841
- Language
- eng
- Reviewed
List Of Titles
