Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/87390
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- Title
- Immunohistochemical assessment of cyclic guanosine monophosphate (cGMP) and soluble guanylate cyclas (sGC) within the rostral ventrolateral medulla
- Related
- Journal of biomedical science, Vol. 15, Issue 6, p.801-812
- DOI
- 10.1007/s11373-008-9269-4
- Publisher
- BioMed Central
- Date
- 2008
- FoR/RFCD Code(s)
-
110000 Medical And Health Sciences
- Author/Creator
- Powers-Martin, Kellysan
- Author/Creator
- Barron, Anna M
- Author/Creator
- Auckland, Clare H
- Author/Creator
- McCooke, John K
- Author/Creator
- McKitrick, Douglas J
- Author/Creator
- Arnolda, Leonard F
- Author/Creator
- Phillips, Jacqueline K
- Description
- Functional evidence suggests that nitric oxide (NO) signalling in the rostral ventrolateral medulla (RVLM) is cGMP-dependent and that this pathway is impaired in hypertension. We examined cGMP expression as a marker of active NO signalling in the C1 region of the RVLM, comparing adult (>18 weeks) Wistar–Kyoto (WKY, n = 4) and spontaneously hypertensive rats (SHR, n = 4). Double label immunohistochemistry for cGMPimmunoreactivity (IR) and C1 neurons [as identified by phenylethanolamine N-methyltransferase (PNMT-IR) or tyrosine hydroxylase TH-IR)], or neuronal NO synthase (nNOS) neurones, failed to reveal cGMP-IR neurons in the RVLM of either strain, despite consistent detection of cGMP-IR in the nucleus ambiguus (NA). This was unchanged in the presence of isobutylmethylxanthine (IBMX; 0.5 mM, WKY, n = 4, SHR n = 2) and in young animals (WKY, 10-weeks, n = 3). Incubation of RVLMslices (WKY, 10-weeks, n = 9) in DETA-NO (100 μm; 10 min) or NMDA (10 μM; 2 min) did not uncover cGMPIR. In all studies, cGMP was prominent within the vasculature. Soluble guanylate cyclase (sGC)-IR was found throughout neurones of the RVLM, but did not co-localise with PNMT, TH or nNOS-IR neurons (WKY, 10-weeks, n = 6). Results indicate that within the RVLM, cGMP is not detectable using immunohistochemistry in the basal state and cannot be elicited by phosphodiesterase inhibition, NMDA receptor stimulation or NO donor application.
- Description
- 12 page(s)
- Subject Keyword
- 110000 Medical And Health Sciences
- Subject Keyword
- RVLM
- Subject Keyword
- immunohistochemistry
- Subject Keyword
- slice preparation
- Subject Keyword
- nitric oxide
- Subject Keyword
- NMDA
- Subject Keyword
- C1 cell group
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian School of Advanced Medicine
- Identifier
- http://hdl.handle.net/1959.14/87390
- Identifier
- ISSN:1021-7770
- Identifier
- mq-rm-2009003867
- Language
- eng
- Rights
- Version archived for private and non-commercial use with the permission of the author and according to publisher conditions. For further rights please contact the publisher.
- Full Text
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