Degenerate oligonucleotide gene shuffling | Macquarie University ResearchOnline

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/86294

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Title: Degenerate oligonucleotide gene shuffling
Date: 2004
Author/Creator: Gibbs, Moreland David
Author/Creator: Bergquist, Peter Leonard
Author/Creator: Nevalainen, Kaisu Milja Helena
Description: A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.
Resource Type: patent
Organisation: Macquarie University. Dept. of Biological Sciences
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

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