Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/69736
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- Title
- Energy transfer in reconstituted peridinin-chlorophyll-protein complexes : ensemble and single-molecule spectroscopy studies
- Related
- Biophysical journal, Vol. 93, Issue 9, p.3249-3258
- DOI
- 10.1529/biophysj.107.112094
- Publisher
- Biophysical Society
- Date
- 2007
- FoR/RFCD Code(s)
-
030606 Structural Chemistry and Spectroscopy
060101 Analytical Biochemistry
- Author/Creator
- Mackowski, Sebastian
- Author/Creator
- Wörmke, Stephan
- Author/Creator
- Brotosudarmo, Tatas H. P
- Author/Creator
- Jung, Christophe
- Author/Creator
- Hiller, Roger G
- Author/Creator
- Scheer, Hugo
- Author/Creator
- Bräuchle, Christoph
- Description
- We combine ensemble and single-molecule spectroscopy to gain insight into the energy transfer between chlorophylls (Chls) in peridinin-chlorophyll-protein (PCP) complexes reconstituted with Chl a, Chl b, as well as both Chl a and Chl b. The main focus is the heterochlorophyllous system (Chl a/b-N-PCP), and reference information essential to interpret experimental observations is obtained from homochlorophyllous complexes. Energy transfer between Chls in Chl a/b-N-PCP takes place from Chl b to Chl a and also from Chl a to Chl b with comparable Förster energy transfer rates of 0.0324 and 0.0215ps⁻¹, respectively. Monte Carlo simulations yield the ratio of 39:61 for the excitation distribution between Chl a and Chl b, which is larger than the equilibrium distribution of 34:66. An average Chl a/Chl b fluorescence intensity ratio of 66:34 is measured, however, for single Chl a/b-N-PCP complexes excited into the peridinin (Per) absorption. This difference is attributed to almost three times more efficient energy transfer from Per to Chl a than to Chl b. The results indicate also that due to bilateral energy transfer, the Chl system equilibrates only partially during the excited state lifetimes.
- Description
- 10 page(s)
- Subject Keyword
- 030606 Structural Chemistry and Spectroscopy
- Subject Keyword
- 060101 Analytical Biochemistry
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Biological Sciences
- Identifier
- http://hdl.handle.net/1959.14/69736
- Identifier
- ISSN:1542-0086
- Identifier
- mq-rm-2007003122
- Language
- eng
- Reviewed
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