Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/69616
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- Title
- Proteomic analysis of early lactation milk of the tammar wallaby (Macropus eugenii)
- Related
- Comparative biochemistry and physiology D : genomics and proteomics, Vol. 2, Issue 2, p.150-164
- DOI
- 10.1016/j.cbd.2007.02.002
- Publisher
- Elsevier
- Date
- 2007
- FoR/RFCD Code(s)
-
030101 Analytical Spectrometry
- Author/Creator
- Joss, Janice
- Author/Creator
- Molloy, Mark
- Author/Creator
- Hinds, Lyn
- Author/Creator
- Deane, Elizabeth
- Description
- We report the successful use of 2D electrophoresis, MALDI MS/MS and chemical derivatisation protocols of guanidination and sulfonation to identify over 100 protein spots present in early marsupial milk (tammar wallaby) at 40 days lactation, where a limited translated genomic database is publicly available for cross species matching and protein identification. Of the proteins identified, 25 matched to 6 existing marsupial milk protein sequences in the NCBI database; another 6 were identified with high confidence to other mammals and have not previously been identified in marsupial milk. By using chemical derivatisation, the reliable identification of a further 81 proteins was achieved. The identified proteins could be grouped into three main functional categories — transport, nutrition and immune protection. All these proteins play a potential role in determining growth and immunological protection of the highly altricial marsupial young at 40 days after birth.
- Description
- 15 page(s)
- Subject Keyword
- 030101 Analytical Spectrometry
- Subject Keyword
- marsupials
- Subject Keyword
- milk
- Subject Keyword
- de novo sequencing
- Subject Keyword
- SPITC
- Subject Keyword
- guanidination
- Subject Keyword
- matrix assisted laser desorption ionization
- Subject Keyword
- two-dimensional gel electrophoresis
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Biological Sciences
- Identifier
- http://hdl.handle.net/1959.14/69616
- Identifier
- ISSN:1744-117X
- Identifier
- mq-rm-2007002755
- Language
- eng
- Reviewed
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