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Assembling the core protein complex of RNA processing events

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/45515

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Title: Assembling the core protein complex of RNA processing events
Related: ComBio2006 Conference (24 - 28 September 2006 : Brisbane)
Related: ComBio 2006 : combined conference abstracts, p.166
Related: Proceedings of the Australian Society for Biochemistry and Molecular Biology Vol. 38
Publisher: Kent Town, SA : Australian Society for Biochemistry and Molecular Biology
Date: 2006
FoR/RFCD Code(s): 270199 Biochemistry and Cell Biology not elsewhere classified
Author/Creator: Sobti, M
Author/Creator: Haynes, P. A
Author/Creator: Kornfeld, G
Author/Creator: Mabbutt, B. C
Description: The LSm proteins are known to organize as multiprotein ring assemblies which interact with RNAs to allow chemical modification or nuclease protection. The determination of structures of large heterogenous protein complexes poses particular difficulty for structural biologists, requiring the careful assembly of components into a viable complex suitable for data collection. Avenues for in vitro assembly include recombinant construction of all or some components via co expression, or expression of polyproteins with engineered linkers. We describe our polyprotein construction of appropriate dimer, trimer and heptamer combinations known to make up mixed LSm subcomplexes. By reconstituting mixed complexes of LSms we are defining the heteroheptameric ring assemblies by crystallography and studying their specific interactions their associated protein factors.
Description: 1 page(s)
Subject Keyword: 270199 Biochemistry and Cell Biology not elsewhere classified
Resource Type: conference paper abstract
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/45515
Identifier: ISSN:1328-4924
Identifier: mq-rm-2006000594
Language: eng

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