Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/43862
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- Title
- Genistein-induced proteome changes in the human endometrial carcinoma cell line, Ishikawa
- Related
- Clinical proteomics, Vol. 2, Issue 3-4, p.153-167
- DOI
- 10.1007/BF02752498
- Publisher
- Humana Press Inc
- Date
- 2007
- FoR/RFCD Code(s)
-
030405 Molecular Medicine
060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
- Author/Creator
- Konstantakopoulos, Nicki
- Author/Creator
- Larsen, Martin R
- Author/Creator
- Campbell, Ian G
- Author/Creator
- Quinn, Michael A
- Author/Creator
- Baker, Mark S
- Author/Creator
- Georgiou, Harry M
- Author/Creator
- Rice, Greg E
- Description
- Epidemiological studies have shown that Asian populations display a lower incidence of hormone-dependant cancers, cardiovascular disease, osteoporosis, and menopausal ailments compared to Western societies. Available data support the proposal that lower incidence is associated with the high dietary consumption of isoflavones, such as genistein. This study used two-dimensional electrophoresis to characterize the effect of genistein on the proteome of an endometrial tumor cell model, namely the Ishikawa cell line. Proteome maps displaying approx 1800 proteins were obtained from cells treated with vehicle or genistein at physiologically attainable concentrations of 0.5, 5, or 50 μM or supra-physiological concentration, 500 μM. The effects of genistein on protein expression were characterized using image analysis software. A total 65 protein spots displayed a significant decrease in expression and 32 proteins displayed a significant increase in expression. Of these protein spots, 29 were randomly selected for characterization by matrix assisted laser desorption/ionization tandem mass spectrometry, yielding 18 different proteins. This type of analysis enabled the characterization of a wide range of cellular proteins and allowed for the identification of functional and biochemical pathways that may be regulated or affected by genistein, including cellular transcription, cell proliferation, stress response, or modulation of oncogenic pathways.
- Description
- 15 page(s)
- Subject Keyword
- 030405 Molecular Medicine
- Subject Keyword
- 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
- Subject Keyword
- Genistein
- Subject Keyword
- endometrial cancer
- Subject Keyword
- proteomics
- Subject Keyword
- phytoestrogen
- Subject Keyword
- tumor suppression
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian Proteome Analysis Facility (APAF)
- Identifier
- http://hdl.handle.net/1959.14/43862
- Identifier
- ISSN:1559-0275
- Identifier
- mq-rm-2007002141
- Language
- eng
- Reviewed
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