Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/42676
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- Title
- Proteins protect lipid membranes from oxidation by thiyl radicals
- Related
- Archives of biochemistry and biophysics, Vol. 459, Issue 2, p.151-158
- DOI
- 10.1016/j.abb.2007.01.016
- Publisher
- Elsevier
- Date
- 2007
- FoR/RFCD Code(s)
-
030501 Free Radical Chemistry
060101 Analytical Biochemistry
- Author/Creator
- Tweeddale, Helen J
- Author/Creator
- Kondo, Mari
- Author/Creator
- Gebicki, Janusz M
- Description
- Oxidation of polyunsaturated fatty acids by thiyl radicals derived from GSH or Cys is believed to be responsible for some of the biological damage resulting from lipid oxidation under oxidative stress. However, this has not been demonstrated in complex biological systems. In this study, we measured the formation of lipid hydroperoxides in liposomes exposed to radicals generated by γ radiation from GSH, GSSG, GSMe, Cys and Met. In the absence of proteins, the radicals oxidized the liposome lipids. In the presence of proteins, the thiyl radicals failed to react with the liposomes, even though the protein radicals efficiently oxidized the S-compounds. It appears that the thiyl and other S-radicals were effectively scavenged by the protein before initiating lipid oxidation. The results suggest that membrane lipid oxidation in vivo by thiyl radicals is unlikely to be a significant event.
- Description
- 8 page(s)
- Subject Keyword
- 030501 Free Radical Chemistry
- Subject Keyword
- 060101 Analytical Biochemistry
- Subject Keyword
- liposomes
- Subject Keyword
- glutathione
- Subject Keyword
- lysozyme
- Subject Keyword
- oxidation
- Subject Keyword
- hydroxyl radical
- Subject Keyword
- thiyl radical
- Subject Keyword
- protein radical
- Subject Keyword
- membrane
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/42676
- Identifier
- ISSN:0003-9861
- Identifier
- mq-rm-2007003030
- Language
- eng
- Reviewed
