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Subcellular shotgun proteomics in plants : looking beyond the usual suspects

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/42588

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Title: Subcellular shotgun proteomics in plants : looking beyond the usual suspects
Related: Proteomics, Vol. 7, Issue 16, p.2963-2975
DOI: 10.1002/pmic.200700216
Publisher: Wiley
Date: 2007
FoR/RFCD Code(s): 030403 Characterisation of Biological Macromolecules 030406 Proteins and Peptides 060702 Plant Cell and Molecular Biology
Author/Creator: Haynes, Paul A
Author/Creator: Roberts, Thomas H
Description: In this review we examine the current state of analytical methods used for shotgun proteomics experiments in plants. The rapid advances in this field in recent years are discussed, and contrasted with experiments performed using current widely used procedures. We also examine the use of subcellular fractionation approaches as they apply to plant proteomics, and discuss how appropriate sample preparation can produce a great increase in proteome coverage in subsequent analysis. We conclude that the conjunction of these two techniques represents a significant advance in plant proteomics, and the future of plant biology research will continue to be enriched by the ongoing development of proteomic analytical technology
Description: 13 page(s)
Subject Keyword: 030403 Characterisation of Biological Macromolecules
Subject Keyword: 030406 Proteins and Peptides
Subject Keyword: 060702 Plant Cell and Molecular Biology
Subject Keyword: liquid chromatography-tandem mass spectrometry
Subject Keyword: rice
Subject Keyword: sample preparation
Subject Keyword: shotgun proteomics
Subject Keyword: subcellular fractionation
Resource Type: journal article
Organisation: Macquarie University. Dept. of Biological Sciences
Organisation: Macquarie University. Australian Proteome Analysis Facility (APAF)

Identifier: http://hdl.handle.net/1959.14/42588
Identifier: ISSN:1615-9861
Identifier: mq-rm-2007001498
Language: eng
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