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Activity-based identification of secreted serine proteases of the filamentous fungus, Ophiostoma

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/42374

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Title: Activity-based identification of secreted serine proteases of the filamentous fungus, Ophiostoma
Related: Biotechnology letters, Vol. 29, No. 6, p.937-943
DOI: 10.1007/s10529-007-9333-6
Publisher: Springer
Date: 2007
FoR/RFCD Code(s): 030401 Biologically Active Molecules 030503 Organic Chemical Synthesis 030599 Organic Chemistry not elsewhere classified
Author/Creator: Wu, Caiyan
Author/Creator: Xu, Qiang
Author/Creator: Liu, Fei
Author/Creator: Nevalainen, K. M. Helena
Description: A general activity probe was synthesized and applied to the supernatant of a filamentous fungus, Ophiostoma, culture to identify directly the secreted serine proteases by covalent enzyme labeling. The activity probe contained a chemically reactive group that reacted with, and thus covalently labeled, the serine residues of only active proteases and not heat-inactivated proteases. The activity probe also contained a fluorescent group that allowed for the subsequent visualization of the captured proteases in 1-D gels and their identification by N-terminal sequencing. This use of a chemical probe led to the rapid discovery of subtilisin-like serine protease of Ophiostoma. Two hypothetical proteins were also captured, with one being a probable endopeptidase K type of protease.
Description: 7 page(s)
Subject Keyword: 030401 Biologically Active Molecules
Subject Keyword: 030503 Organic Chemical Synthesis
Subject Keyword: 030599 Organic Chemistry not elsewhere classified
Subject Keyword: enzyme labeling
Subject Keyword: filamentous fungi
Subject Keyword: fluorescent activity probe
Subject Keyword: Ophiostoma
Subject Keyword: secreted proteases
Resource Type: journal article
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/42374
Identifier: ISSN:1573-6776
Identifier: mq-rm-2007000776
Language: eng
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