Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/42140
66 Visitors
72 Hits
0 Downloads
- Title
- The Development of multiple reaction monitoring assays for liver-derived plasma proteins
- Related
- Proteomics, Vol. 1, Issue 12, p.1570-1581
- DOI
- 10.1002/prca.200700305
- Publisher
- Wiley
- Date
- 2007
- FoR/RFCD Code(s)
-
030499 Medicinal and Biomolecular Chemistry not elsewhere classified
060504 Microbial Ecology
100202 Biological Control
- Author/Creator
- Mckay, Matthew J
- Author/Creator
- Sherman, Jamie
- Author/Creator
- Laver, Matthew T
- Author/Creator
- Baker, Mark S
- Author/Creator
- Clarke, Stephen J
- Author/Creator
- Molloy, Mark P
- Description
- There is wide interpatient variability in toxicity to chemotherapeutic drugs and a lack of routine clinical tests for prospectively identifying patients at risk of developing toxicity from chemotherapy. An empirically driven MS strategy has been developed to monitor liver-derived plasma proteins as potential biomarkers of early toxicity. Multiple reaction monitoring (MRM) has been used to assess 46 candidate peptides from 18 liver-derived proteins. Following an iterative process of assay design, optimisation and assessment we selected 29 MRM assays (median CV 4.6%, range 1.2-11.6%) and monitored changes in levels of plasma proteins from a small number of colorectal cancer (CRC) patients undergoing chemotherapy. We demonstrated MRM assay robustness, and show that patients undergo minor elevation in plasma proteins when profiled on Day 3 of the chemotherapeutic regime. The MRM assays were in general agreement with 2-D DIGE-based quantitation from the same patient samples. The data supports the application of MRM-based methods as facile, highly reproducible, medium-throughput techniques that warrant expanded investigation for clinical utility in identifying patients at risk of developing chemotoxicity.
- Description
- 12 page(s)
- Subject Keyword
- 030499 Medicinal and Biomolecular Chemistry not elsewhere classified
- Subject Keyword
- 060504 Microbial Ecology
- Subject Keyword
- 100202 Biological Control
- Subject Keyword
- biomarker
- Subject Keyword
- colorectal cancer
- Subject Keyword
- Multiple reaction monitoring (MRM)
- Subject Keyword
- quantitation
- Subject Keyword
- tandem mass spectrometry
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian Proteome Analysis Facility (APAF)
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Organisation
- Macquarie University. Dept. of Philosophy
- Identifier
- http://hdl.handle.net/1959.14/42140
- Identifier
- ISSN:1615-9861
- Identifier
- mq-rm-2007002876
- Language
- eng
- Reviewed
List Of Titles
