Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection | Macquarie University ResearchOnline

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/42023

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Title: Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection
Related: Biotechnology letters, Vol. 29, Issue 12, p.1831-1837
DOI: 10.1007/s10529-007-9487-2
Publisher: Springer
Date: 2007
Author/Creator: Ferrari, B. C
Author/Creator: Power, M. L
Author/Creator: Bergquist, P. L
Description: Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.
Description: 7 page(s)
Subject Keyword: DNA extraction
Subject Keyword: Cryptosporidium
Subject Keyword: Giardia
Subject Keyword: Haemonchus
Subject Keyword: Nematoda
Subject Keyword: proteinase
Subject Keyword: protozoa
Resource Type: journal article
Organisation: Macquarie University. Dept. of Biological Sciences
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

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