Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/41936
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- Title
- Two dimensional differential in-gel electrophoresis of leaf and roots of lycopersicon esculentum
- Related
- Thiellement, Hervé; Zivy, Michel; Damerval, Catherine and Méchin, Valérie. Plant proteomics : methods and protocols, p.157-174
- DOI
- 10.1385/1-59745-227-0:157
- Related
- Methods in molecular biology 355
- Publisher
- Totowa, NJ : Humana Press
- Date
- 2006
- FoR/RFCD Code(s)
-
030108 Separation Science
030199 Analytical Chemistry not elsewhere classified
060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
- Author/Creator
- Keeler, Matthew
- Author/Creator
- Letarte, Jessica
- Author/Creator
- Hattrup, Emily
- Author/Creator
- Hickman, Fatimah
- Author/Creator
- Haynes, Paul, A
- Description
- In this report we present a detailed protocol for the analysis of differential protein expression between two plant tissue samples. The protocol involves harvesting of leaves and roots from mature tomato plants, preparing protein extracts from the harvested tissues, fluorescent labeling of each sample prior to differential in-gel electrophoresis (DIGE), first- and second-dimension electrophoretic separations, and image analysis to visualize and quantify differential protein expression. This protocol is adaptable for use with a wide variety of plant materials and can be used to measure protein expression changes occurring in response to abiotic stress, biotic stress, genetic manipulation, selective breeding, and many other conditions. In addition to the detailed protocol, we also present the results of a representative experiment analyzing subtle changes in protein expression in the roots of tomato plants grown under control and salt-stress conditions.
- Subject Keyword
- 030108 Separation Science
- Subject Keyword
- 030199 Analytical Chemistry not elsewhere classified
- Subject Keyword
- 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
- Subject Keyword
- 2D protein gel electrophoresis
- Subject Keyword
- differential in-gel electrophoresis
- Subject Keyword
- DIGE
- Subject Keyword
- plant protein
- Subject Keyword
- tomato
- Subject Keyword
- CyDye protein staining
- Subject Keyword
- differential wavelength fluorescence imaging
- Subject Keyword
- lycopersicon esculentum
- Resource Type
- book chapter
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/41936
- Identifier
- ISBN:9781588296351
- Identifier
- mq-rm-2007003083
- Language
- eng
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