The Neyriz ophiolite along the northeast flank of the Zagros fold-thrust belt in southern Iran is an excellent example of a Late Cretaceous supra-subduction zone (SSZ)-related ophiolite on the north side of the Neotethys. The ophiolite comprises a mantle sequence including lherzolite, harzburgite, diabasic dikes, and cumulate to mylonitic gabbro lenses, and a crustal sequence comprising a sheeted dike complex and pillow lavas associated with pelagic limestone and radiolarite. Mantle harzburgites contain less CaO and Al₂O₃, are depleted in rare earth elements, and contain spinels that are more Cr-rich than lherzolites. Mineral compositions of peridotites are similar to those of both abyssal and SSZ- peridotites. Neyriz gabbroic rocks show boninitic (SSZ-related) affinities, while crustal rocks are similar to early arc tholeiites. Mineral compositions of gabbroic rocks resemble those of SSZ-related cumulates such as high forsterite olivine, anorthite-rich plagioclase, and high-Mg# clinopyroxene. Initial εNd(t) values range from +7.9 to +9.3 for the Neyriz magmatic rocks. Samples with radiogenic Nd overlap with least radiogenic mid-ocean ridge basalts and with Semail and other Late Cretaceous Tethyan ophiolitic rocks. Initial ⁸⁷Sr/⁸⁶Sr ranges from 0.7033 to 0.7044, suggesting modification due to seafloor alteration. Most Neyriz magmatic rocks are characterized by less radiogenic ²⁰⁷Pb/²⁰⁴Pb (near the northern hemisphere reference line), suggesting less involvement of sediments in their mantle source. Our results for Neyriz ophiolite and the similarity to other Iranian Zagros ophiolites support a subduction initiation setting for its generation.