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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/357979
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- Title
- Quantitative proteomic analysis of paired colorectal cancer and non-tumorigenic tissues reveals signature proteins and perturbed pathways involved in CRC progression and metastasis
- Related
- Journal of proteomics, Vol. 126, (2015), p.54-67
- DOI
- 10.1016/j.jprot.2015.05.037
- Publisher
- Elsevier
- Date
- 2015
- Author/Creator
- Sethi, Manveen K
- Author/Creator
- Thaysen-Andersen, Morten
- Author/Creator
- Kim, Hoguen
- Author/Creator
- Park, Cheol Keun
- Author/Creator
- Baker, Mark S
- Author/Creator
- Packer, Nicolle H
- Author/Creator
- Paik, Young-Ki
- Author/Creator
- Hancock, William S
- Author/Creator
- Fanayan, Susan
- Description
- Modern proteomics has proven instrumental in our understanding of the molecular deregulations associated with the development and progression of cancer. Herein, we profile membrane-enriched proteome of tumor and adjacent normal tissues from eight CRC patients using label-free nanoLC-MS/MS-based quantitative proteomics and advanced pathway analysis. Of the 948 identified proteins, 184 proteins were differentially expressed (P<0.05, fold change>1.5) between the tumor and non-tumor tissue (69 up-regulated and 115 down-regulated in tumor tissues). The CRC tumor and non-tumor tissues clustered tightly in separate groups using hierarchical cluster analysis of the differentially expressed proteins, indicating a strong CRC-association of this proteome subset. Specifically, cancer associated proteins such as FN1, TNC, DEFA1, ITGB2, MLEC, CDH17, EZR and pathways including actin cytoskeleton and RhoGDI signaling were deregulated. Stage-specific proteome signatures were identified including up-regulated ribosomal proteins and down-regulated annexin proteins in early stage CRC. Finally, EGFR⁺ CRC tissues showed an EGFR-dependent down-regulation of cell adhesion molecules, relative to EGFR⁻ tissues. Taken together, this study provides a detailed map of the altered proteome and associated protein pathways in CRC, which enhances our mechanistic understanding of CRC biology and opens avenues for a knowledge-driven search for candidate CRC protein markers.
- Description
- 14 page(s)
- Subject Keyword
- Colorectal cancer
- Subject Keyword
- Membrane proteins
- Subject Keyword
- Label free shotgun proteomics
- Subject Keyword
- Epidermal growth factor receptor
- Subject Keyword
- Fibronectin
- Subject Keyword
- Malectin
- Resource Type
- journal article
- Organisation
- Macquarie University. Department of Chemistry and Biomolecular Sciences
- Organisation
- Macquarie University. Department of Biomedical Sciences
- Identifier
- http://hdl.handle.net/1959.14/357979
- Identifier
- mq:40082
- Identifier
- ISSN:1874-3919
- Identifier
- mq-rm-2013012853
- Identifier
- mq_res-se-131562
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
