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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/272273
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- Title
- Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites
- Related
- Marine drugs, Vol. 11, No. 1, (2013), p.40-49
- DOI
- 10.3390/md11010040
- Publisher
- Molecular Diversity Preservation International (MDPI)
- Date
- 2013
- Author/Creator
- Penesyan, Anahit
- Author/Creator
- Ballestriero, Francesco
- Author/Creator
- Daim, Malak
- Author/Creator
- Kjelleberg, Staffan
- Author/Creator
- Thomas, Torsten
- Author/Creator
- Egan, Suhelen
- Description
- A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.
- Description
- 10 page(s)
- Subject Keyword
- 111500 Pharmacology and Pharmaceutical Sciences
- Subject Keyword
- Antibacterial
- Subject Keyword
- Antinematode
- Subject Keyword
- Caenorhabditis elegans
- Subject Keyword
- Fosmid libraries
- Subject Keyword
- Functional genomic screen
- Subject Keyword
- Marine bacteria
- Subject Keyword
- Marine bioactives
- Resource Type
- journal article
- Organisation
- Macquarie University. Department of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/272273
- Identifier
- mq:28798
- Identifier
- ISSN:1660-3397
- Identifier
- mq-rm-2012001457
- Identifier
- mq_res-ext-2-s2.0-84873118613
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
