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Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/188975

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Title: Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus
Related: Letters in applied microbiology, Vol. 54, Issue 3, (2012), p.263-266
DOI: 10.1111/j.1472-765X.2011.03197.x
Publisher: Wiley-Blackwell Publishing
Date: 2012
Author/Creator: Lawson, T. S
Author/Creator: Connally, R. E
Author/Creator: Vemulpad, S
Author/Creator: Piper, J. A
Description: Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.
Description: 4 page(s)
Subject Keyword: Detection
Subject Keyword: Identification
Subject Keyword: Infection
Subject Keyword: Rapid methods
Subject Keyword: Staphylococci
Resource Type: journal article
Organisation: Macquarie University. Dept. of Physics and Astronomy
Organisation: Macquarie University. Dept. of Chiropractic
Organisation: Macquarie University. Office of the Deputy Vice-Chancellor (Research)

Identifier: http://hdl.handle.net/1959.14/188975
Identifier: ISSN:0266-8254
Identifier: mq_res-ext-2-s2.0-84856955767
Language: eng
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