Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/188941
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- Title
- The "Atheroprotective" mediators apolipoproteinA-I and Foxp3 are over-abundant in unstable carotid plaques
- Related
- International journal of cardiology, Vol. 145, Issue 2, (2010), p.183-187
- DOI
- 10.1016/j.ijcard.2009.05.024
- Publisher
- Elsevier Ireland
- Date
- 2010
- FoR/RFCD Code(s)
-
110200 Cardiovascular Medicine and Haematology
- Author/Creator
- Patel, S
- Author/Creator
- Chung, S. H
- Author/Creator
- White, G
- Author/Creator
- Bao, S
- Author/Creator
- Celermajer, D. S
- Description
- Objective - Inflammation is important in plaque vulnerability but the role of atheroprotective mediators in unstable plaques is not defined. The apolipoproteinA-I (apoA-I) component of HDL, and CD4+/CD25+ regulatory T cells (with their major transcription factor, Foxp3), have been implicated in the suppression of vascular inflammation. Our aim was to characterise the presence of these novel “protective” markers (apoA-I and Foxp3) in carotid plaques from symptomatic and asymptomatic subjects. Methods and results - Plaques from 57 patients (25 symptomatic, 32 asymptomatic) were stained immunohistochemically for macrophages (CD68), T cells (CD3), monocyte chemotactic protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), myeloperoxidase (MPO), apoA-I and Foxp3. Twelve randomly selected plaques (6 asymptomatic, 6 symptomatic) were immunostained for interleukin-10 (IL-10) and interleukin-17 (IL-17). Staining was quantified using Image-Pro Plus software. Significantly greater areas of positive immunostaining for CD68, CD3, MCP-1, MMP-2, IL-17 and MPO were found in plaques from symptomatic patients compared with asymptomatic patients (p < 0.05 for all). Furthermore, significantly greater areas of positive immunostaining for apoA-I, Foxp3 and IL-10 were found in symptomatic versus asymptomatic plaques (p < 0.05 for all). The presence of apoA-I was correlated significantly and co-localised with CD3, CD68, MCP-1, MMP-2 and MPO immunostaining (R = 0.70, 0.63, 0.52, 0.55 and 0.79, respectively; p < 0.01 for all). Foxp3 immunostaining also correlated significantly with CD3 (R = 0.42), CD68 (R = 0.47), MCP-1 (R = 0.55) and MMP-2 (R = 0.44) immunostaining (p < 0.05 for all). Conclusions - ApoA-I and Foxp3 are over-abundant in plaques from symptomatic subjects and co-localise with key inflammatory mediators. These data suggest ineffective/insufficient protection against atherosclerosis-mediated inflammation by these “atheroprotective” moieties.
- Description
- 5 page(s)
- Subject Keyword
- 110200 Cardiovascular Medicine and Haematology
- Subject Keyword
- Atherosclerosis
- Subject Keyword
- Inflammation
- Subject Keyword
- ApolipoproteinA-I
- Subject Keyword
- Foxp3
- Subject Keyword
- T cells
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian School of Advanced Medicine
- Identifier
- http://hdl.handle.net/1959.14/188941
- Identifier
- ISSN:0167-5273
- Identifier
- mq-rm-2011003365
- Language
- eng
- Reviewed
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