Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging | Macquarie University ResearchOnline

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Title: Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging
Related: Optics express, Vol. 20, No. 13, (2012), p.14100-14108
DOI: 10.1364/OE.20.014100
Publisher: Optical Society of America
Date: 2012
Author/Creator: Ding, Yichen
Author/Creator: Xie, Hao
Author/Creator: Peng, Tong
Author/Creator: Lu, Yiqing
Author/Creator: Jin, Dayong
Author/Creator: Teng, Junlin
Author/Creator: Ren, Qiushi
Author/Creator: Xi, Peng
Description: The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens.
Description: 9 page(s)
Resource Type: journal article
Organisation: Macquarie University. MQ Photonics Research Centre

Identifier: http://hdl.handle.net/1959.14/184410
Identifier: ISSN:1094-4087
Identifier: mq_res-ext-2-s2.0-84863759369
Language: eng
Rights: This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-13-14100]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
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