Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/183086
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- Title
- Achieving λ/10 resolution CW STED nanoscopy with a Ti : Sapphire oscillator
- Related
- PLoS ONE, Vol. 7, Issue 6, Article e40003, (2012), p.1-9
- DOI
- 10.1371/journal.pone.0040003
- Publisher
- Public Library of Science
- Date
- 2012
- Author/Creator
- Liu, Yujia
- Author/Creator
- Ding, Yichen
- Author/Creator
- Alonas, Eric
- Author/Creator
- Zhao, Wenli
- Author/Creator
- Santangelo, Philip J
- Author/Creator
- Jin, Dayong
- Author/Creator
- Piper, James A
- Author/Creator
- Teng, Junlin
- Author/Creator
- Ren, Qiushi
- Author/Creator
- Xi, Peng
- Description
- In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STE D over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED.
- Description
- 9 page(s)
- Resource Type
- journal article
- Organisation
- Macquarie University. MQ Photonics Research Centre
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/183086
- Identifier
- ISSN:1932-6203
- Identifier
- mq-rm-2011008271
- Identifier
- mq_res-ext-2-s2.0-84862995094
- Language
- eng
- Rights
- Copyright the Author(s) [2012]. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
- Full Text
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