Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/180371
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- Title
- Galanin is a selective marker of the retrotrapezoid nucleus in rats
- Related
- Journal of comparative neurology, Vol. 512, Issue 3, (2009), p.373-383
- DOI
- 10.1002/cne.21897
- Publisher
- Wiley-Blackwell Publishing
- Date
- 2009
- FoR/RFCD Code(s)
-
110900 Neurosciences
- Author/Creator
- Stornetta, Ruth L
- Author/Creator
- Spirovski, Darko
- Author/Creator
- Moriera, Thiago S
- Author/Creator
- Takakura, Ana C
- Author/Creator
- West, Gavin H
- Author/Creator
- Gwilt, Justin M
- Author/Creator
- Pilowsky, Paul M
- Author/Creator
- Guyenet, Patrice G
- Description
- The rat retrotrapezoid nucleus (RTN) contains CO₂-activated neurons that contribute to the central chemoreflex and to breathing automaticity. These neurons have two known markers, the transcription factor Phox2b and vesicular glutamate transporter 2 (VGLUT2). Noncatecholaminergic galanin-immunoreactive (ir) neurons within a region of the lower brainstem that seems identical to what is currently defined as the RTN have been previously described. Here we ask whether these galanin-expressing neurons are the same cells as the recently characterized CO₂-sensitive neurons of the RTN. By using in situ hybridization, we found that pre-pro-galanin (PPGal) mRNA is expressed by an isolated cluster of neurons that is co-extensive with the RTN as defined by a population of strongly Phox2b-ir neurons devoid of tyrosine hydroxylase (Phox2b⁺/TH⁻ neurons). This bilateral structure contains about 1,000 PPGal mRNA-positive neurons in the rat. The PPGal mRNA-positive neurons were Phox2b⁺/TH⁻ and as susceptible to destruction by the toxin [Sar⁹, Met (O₂)¹¹]-substance P as the rest of the RTN Phox2b⁺/TH⁻ cells of the RTN. CO₂-activated neurons were recorded in the RTN of anesthetized rats and were labeled with biotinamide. Many of those cells (7/17, 41%, five rats) contained PPGal-mRNA. In conclusion, galanin mRNA is a very specific marker of the glutamatergic Phox2b⁺/TH⁻ neurons of the RTN, but galanin mRNA identifies only half of these putative central respiratory chemoreceptors.
- Description
- 11 page(s)
- Subject Keyword
- 110900 Neurosciences
- Subject Keyword
- central chemoreceptors
- Subject Keyword
- retrotrapezoid nucleus
- Subject Keyword
- galanin
- Subject Keyword
- medulla oblongata
- Subject Keyword
- Phox2b
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian School of Advanced Medicine
- Identifier
- http://hdl.handle.net/1959.14/180371
- Identifier
- ISSN:0021-9967
- Identifier
- mq-rm-2007009365
- Language
- eng
- Reviewed
