Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/178940
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- Title
- The Effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5
- Related
- PLoS ONE, Vol. 7, Issue 6, Article e39139, (2012),
- DOI
- 10.1371/journal.pone.0039139
- Publisher
- Public Library of Science
- Date
- 2012
- Author/Creator
- Lim, Chee Kent
- Author/Creator
- Hassan, Karl A
- Author/Creator
- Tetu, Sasha G
- Author/Creator
- Loper, Joyce E
- Author/Creator
- Paulsen, Ian T
- Description
- One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels.
- Description
- 18 page(s)
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/178940
- Identifier
- ISSN:1932-6203
- Identifier
- mq_res-ext-2-s2.0-84862496171
- Language
- eng
- Rights
- Copyright the Author(s) [2012]. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
- Full Text
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