Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/176090
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- Title
- Developing red-emissive ruthenium(II) complex-based luminescent probes for cellular imaging
- Related
- Bioconjugate chemistry, Vol. 23, No. 4, (2012), p.725-733
- DOI
- 10.1021/bc200506w
- Publisher
- American Chemical Society
- Date
- 2012
- Author/Creator
- Zhang, Run
- Author/Creator
- Ye, Zhiqiang
- Author/Creator
- Yin, Yuejiao
- Author/Creator
- Wang, Guilan
- Author/Creator
- Jin, Dayong
- Author/Creator
- Yuan, Jingli
- Author/Creator
- Piper, James A
- Description
- (Figure Presented) Ruthenium(II) complexes have rich photophysical attributes, which enable novel design of responsive luminescence probes to selectively quantify biochemical analytes. In this work, we developed a systematic series of Ru(II)- bipyrindine complex derivatives, [Ru(bpy) 3-n(DNP-bpy) n](PF 6) 2 (n = 1, 2, 3; bpy, 2,2′-bipyridine; DNP-bpy, 4-(4-(2,4- dinitrophenoxy)phenyl)-2, 2′-bipyridine), as luminescent probes for highly selective and sensitive detection of thiophenol in aqueous solutions. The specific reaction between the probes and thiophenol triggers the cleavage of the electron acceptor group, 2,4-dinitrophenyl, eliminating the photoinduced electron transfer (PET) process, so that the luminescence of on-state complexes, [Ru(bpy) 3-n(HP-bpy) n] 2+ (n = 1, 2, 3; HP-bpy, -(4-hydroxyphenyl)-2,2′- bipyridine), is turned on. We found that the complex [Ru(bpy)(DNP-bpy) 2] 2+ remarkably enhanced the on-to-off contrast ratio compared to the other two (37.8 compared to 21 and 18.7). This reveals a new strategy to obtain the best Ru(II) complex luminescence probe via the most asymmetric structure. Moreover, we demonstrated the practical utility of the complex as a cell-membrane permeable probe for quantitative luminescence imaging of the dynamic intracellular process of thiophenol in living cells. The results suggest that the new probe could be a very useful tool for luminescence imaging analysis of the toxic thiophenol in intact cells.
- Description
- 9 page(s)
- Resource Type
- journal article
- Organisation
- Macquarie University. MQ Photonics Research Centre
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/176090
- Identifier
- ISSN:1043-1802
- Identifier
- mq-rm-2011007242
- Identifier
- mq_res-ext-2-s2.0-84859950992
- Language
- eng
- Reviewed
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