Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/168724
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- Title
- Hydrogen peroxide detection at a horseradish peroxidase biosensor with a Au nanoparticle-dotted titanate nanotube|hydrophobic ionic liquid scaffold
- Related
- Biosensors and bioelectronics, Vol. 32, No. 1, (2012), p.188-194
- DOI
- 10.1016/j.bios.2011.12.002
- Publisher
- Elsevier BV
- Date
- 2012
- Author/Creator
- Liu, Xiaoqiang
- Author/Creator
- Feng, Heqing
- Author/Creator
- Zhang, Jiamei
- Author/Creator
- Zhao, Ruoxia
- Author/Creator
- Liu, Xiuhua
- Author/Creator
- Wong, Danny K. Y
- Description
- In this work, a novel sensing scaffold, consisting Au nanoparticle (GNP)–dotted TiO₂ nanotubes (TNTs) as the rigid material and the hydrophobic ionic liquid (HIL), 1-decyl-3-methylimidazolium tetrafluoroborate, as the entrapping agent, was applied to facilitate the electron transfer of horseradish peroxidase (HRP) on a glassy carbon electrode. GNPs were immobilised on the TNTs in our work using a one-step reduction of HAuCl₄·3H₂O by sodium borohydride in the presence of sodium citrate as a stabilising reagent. The morphology and composition of the as-synthesised composite materials were characterised by transmission electron microscopy, scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. Cyclic voltammetry of HRP at the modified electrode presented a pair of reproducible, quasi-reversible redox peaks with a peak-to-peak separation of 69 mV, indicating electron transfer between HRP and composite electrode. The GNP–TNT|HIL|HRP electrode was then applied to the detection of H₂O₂ in a pH 7.0 phosphate buffer using chronoamperometry. The biosensor exhibited a linear response in the 15–750 μM range, and a limit of detection of 2.2 μM. The biosensor also exhibited stability with 90% of the detection signal retained over a two-week duration.
- Description
- 7 page(s)
- Subject Keyword
- Gold nanoparticle–dotted TiO₂ nanotubes
- Subject Keyword
- TiO₂–ionic liquid composite materials
- Subject Keyword
- Electron transfer of HRP
- Subject Keyword
- Detection of H₂O₂
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/168724
- Identifier
- ISSN:0956-5663
- Identifier
- mq-rm-2011006196
- Identifier
- mq_res-ext-2-s2.0-84855801431
- Language
- eng
- Reviewed
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