Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/158282
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- Title
- Effect of phospholipid deposits on adhesion of bacteria to contact lenses
- Related
- Optometry and vision science, Vol. 89, Issue 1, (2012), p.52-61
- DOI
- 10.1097/OPX.0b013e318238284c
- Publisher
- Lippincott Williams & Wilkins
- Date
- 2012
- Author/Creator
- Babaei Omali, Negar
- Author/Creator
- Proschogo, Nicholas
- Author/Creator
- Zhu, Hua
- Author/Creator
- Zhao, Zhenjun
- Author/Creator
- Diec, Jennie
- Author/Creator
- Borazjani, Roya
- Author/Creator
- Willcox, Mark D. P
- Description
- PURPOSE.: Protein and lipid deposits on contact lenses may contribute to clinical complications. This study examined the effect of phospholipids on the adhesion of bacteria to contact lenses. METHODS.: Worn balafilcon A (n = 11) and senofilcon A (n = 11) were collected after daily wear and phospholipids were extracted in chloroform:methanol. The amount of phospholipid was measured by electrospray ionization mass spectrometry. Unworn lenses soaked in phospholipids were exposed to Pseudomonas aeruginosa and Staphylococcus aureus. After 18 h incubation, the numbers of P. aeruginosa or S. aureus that adhered to the lenses were measured. Phospholipid was tested for possible effects on bacterial growth. RESULTS.: A broad range of sphingomyelins (SM) and phosphatidylcholines (PC) were detected from both types of worn lenses. SM (16:0) (m/z 703) and PC (34:2) (m/z 758) were the major phospholipids detected in the lens extracts. Phospholipids did not alter the adhesion of any strain of P. aeruginosa or S. aureus (p > 0.05). Phospholipids (0.1 mg/mL) showed no effect on the growth of P. aeruginosa 6294 or S. aureus 031. CONCLUSIONS.: Phospholipids adsorb/absorb to contact lenses during wear, however, the major types of phospholipids adsorbed to lenses do not alter bacterial adhesion or growth.
- Description
- 10 page(s)
- Subject Keyword
- Phospholipid
- Subject Keyword
- Pseudomonas aeruginosa
- Subject Keyword
- Silicone hydrogel contact lenses
- Subject Keyword
- Staphylococcus aureus
- Subject Keyword
- Tear film
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian School of Advanced Medicine
- Identifier
- http://hdl.handle.net/1959.14/158282
- Identifier
- ISSN:1040-5488
- Identifier
- mq_res-20120229-102120
- Language
- eng
- Reviewed
