During first semester of 2002, Macquarie University Library and Department of Biological Sciences conducted a self-assessment audit of biology undergraduate information literacy skills. A survey asking biology academic staff to identify what information literacy skills they expected students to have entering each year of study was also undertaken. The audit revealed that most first year students felt confident in their ability to find and use information resources and that confidence increased over the semester. Despite this confidence, testing at the end of semester demonstrated that the level of information skills of students still varied greatly. The results of the staff survey also highlighted that the type and level of information skills that students were expected to have varied considerably and often depended upon the specialized needs of unit content.