Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/141635
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- Title
- UV LED excited time-gated luminescence flow cytometry : evaluation for rare-event particle counting
- Related
- Imaging, manipulation, and analysis of biomolecules, cells, and tissues (6th : 2008) (21 - 23 January 2008 : San Jose, Calif.)
- Related
- Farkas, Daniel L.; Nicolau, Dan V. and Leif, Robert C.. Imaging, manipulation, and analysis of biomolecules, cells, and tissues VI : 21-23 January 2008, San Jose, California, USA
- DOI
- 10.1117/12.762077
- Related
- SPIE proceedings series Vol. 6859
- Publisher
- Bellingham : SPIE
- Date
- 2008
- Author/Creator
- Jin, Dayong
- Author/Creator
- Ferrari, Belinda
- Author/Creator
- Leif, Robert
- Author/Creator
- Yang, Sean
- Author/Creator
- Vallarino, Lidia M
- Author/Creator
- Williams, John
- Author/Creator
- Piper, James
- Description
- Flow cytometric detection of specific rare-event targets within high-background samples such as water or food are frequently defeated by the extremely large population of non-target background particles. Time-gated detection of long lifetime fluorescence (>10μs) labeled microbial targets has been proven highly efficient in suppressing this non-target autofluorescent (<0.1μs) background. A time-gated luminescence (TGL) flow cytometer using UV LED excitation has demonstrated the successful detection of rare-event particles in high autofluorescence background samples. In this report, high-quality 5μm europium beads were made (homogenous intensity and aggregation free) for a detailed evaluation of the prototype performance. The known number of beads (10±2, 100±20 and 1000±100) were first sorted by a conventional flow cytometry sorter, and spiked into an environmental water concentrate (1 ml; containing >10 million non-target particles). The recovery rate for counting these very-rare-event particles using the TGL flow cytometer was then found to be 100%±20% between bead concentrations evaluated.
- Description
- 11 page(s)
- Resource Type
- conference paper
- Organisation
- Macquarie University. Dept. of Physics
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/141635
- Identifier
- ISBN:9780819470348
- Identifier
- ISSN:0277-786X
- Identifier
- mq-rm-2008000588
- Language
- eng
- Full Text
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