Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/139970
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- Title
- Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin
- Related
- Journal of clinical laboratory analysis, Vol. 25, Issue 2, (2011), p.142-147
- DOI
- 10.1002/jcla.20448
- Publisher
- John Wiley & Sons
- Date
- 2011
- Author/Creator
- Lawson, Thomas S
- Author/Creator
- Connally, Russell E
- Author/Creator
- Iredell, Jonathan R
- Author/Creator
- Vemulpad, Subramanyam
- Author/Creator
- Piper, James A
- Description
- To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1hr protocol is a useful alternative to conventional FISH.
- Description
- 6 page(s)
- Subject Keyword
- Early diagnosis
- Subject Keyword
- Fluorescent in situ hybridization
- Subject Keyword
- Gram-positive bacteria
- Subject Keyword
- Lysostaphin
- Subject Keyword
- Lysozyme
- Subject Keyword
- Molecular diagnostic
- Subject Keyword
- Staphylococcus aureus
- Subject Keyword
- Techniques
- Resource Type
- journal article
- Organisation
- Macquarie University. Faculty of Science
- Organisation
- Macquarie University. Dept. of Physics and Astronomy
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/139970
- Identifier
- ISSN:0887-8013
- Identifier
- mq_res-ext-2-s2.0-79952668948
- Language
- eng
- Reviewed
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