Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/134156
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- Title
- Structural and functional analysis of the type III secretion system from Pseudomonas fluorescens Q8r1-96∇§
- Related
- Journal of bacteriology, Vol. 193, Issue 1, (2011), p.177-189
- DOI
- 10.1128/JB.00895-10
- Publisher
- American Society for Microbiology
- Date
- 2011
- Author/Creator
- Mavrodi, Dimitri V
- Author/Creator
- Joe, Anna
- Author/Creator
- Mavrodi, Olga V
- Author/Creator
- Hassan, Karl A
- Author/Creator
- Weller, David M
- Author/Creator
- Paulsen, Ian T
- Author/Creator
- Loper, Joyce E
- Author/Creator
- Alfano, James R
- Author/Creator
- Thomashow, Linda S
- Description
- Pseudomonas fluorescens Q8r1-96 represents a group of rhizosphere strains responsible for the suppressiveness of agricultural soils to take-all disease of wheat. It produces the antibiotic 2,4-diacetylphloroglucinol and aggressively colonizes the roots of cereal crops. In this study, we analyzed the genome of Q8r1-96 and identified a type III protein secretion system (T3SS) gene cluster that has overall organization similar to that of the T3SS gene cluster of the plant pathogen Pseudomonas syringae. We also screened a collection of 30 closely related P. fluorescens strains and detected the T3SS genes in all but one of them. The Q8r1-96 genome contained ropAA and ropM type III effector genes, which are orthologs of the P. syringae effector genes hopAA1-1 and hopM1, as well as a novel type III effector gene designated ropB. These type III effector genes encoded proteins that were secreted in culture and injected into plant cells by both P. syringae and Q8r1-96 T3SSs. The Q8r1-96 T3SS was expressed in the rhizosphere, but mutants lacking a functional T3SS were not altered in their rhizosphere competence. The Q8r1-96 type III effectors RopAA, RopB, and RopM were capable of suppressing the hypersensitive response and production of reactive oxygen species, two plant immune responses. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
- Description
- 13 page(s)
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/134156
- Identifier
- ISSN:0021-9193
- Identifier
- mq_res-ext-2-s2.0-78650090888
- Language
- eng
- Reviewed
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