Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/130818
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- Title
- Silver and gold nanoparticle coated membranes applied to protein dot blots
- Related
- Journal of nanoparticle research, Vol. 13, Issue 2, (2011), p.613-624
- DOI
- 10.1007/s11051-010-0056-6
- Publisher
- Springer Netherlands
- Date
- 2011
- FoR/RFCD Code(s)
-
091200 Materials Engineering
020200 Atomic, Molecular, Nuclear, Particle and Plasma Physics
100700 Nanotechnology
- Author/Creator
- Xie, F
- Author/Creator
- Drozdowicz-Tomsia, K
- Author/Creator
- Shtoyko, T
- Author/Creator
- Goldys, E. M
- Description
- Detection and identification of low abundance biomarker proteins is frequently based on various types of membrane-based devices. Lowering of the protein detection limits is vital in commercial applications such as lateral flow assays and in Western blots widely used in proteomics. These currently suffer from insufficient detection sensitivity and low retention for small 2–5 kDa proteins. In this study, we report the deposition of two types of metal nanoparticles: gold colloids (50–95 nm diameter) and silver fractals onto a range of commonly used types of membranes including polyvinylidene fluoride (PVDF). Due to strong affinity of proteins to noble metals, such modified membranes have the potential to effectively capture trace proteins preventing their loss. The membranes modified by metal particles were characterized optically and by SEM. The membrane performance in protein dot blots was evaluated using the protein—fluorophore conjugates Deep Purple-bovine serum albumin and fluorescein—human serum albumin. We found that the metal nanoparticles increase light extinction by metals, which is balanced by increased fluorescence, so that the effective fluorescence signal is unchanged. This feature combined with the capture of proteins by the nanoparticles embedded in the membrane increases the detection limit of membrane assays.
- Description
- 12 page(s)
- Subject Keyword
- 091200 Materials Engineering
- Subject Keyword
- 020200 Atomic, Molecular, Nuclear, Particle and Plasma Physics
- Subject Keyword
- 100700 Nanotechnology
- Subject Keyword
- membranes
- Subject Keyword
- noble metal nanoparticles
- Subject Keyword
- electrochemically deposited silver
- Subject Keyword
- dot blot
- Subject Keyword
- protein detection
- Subject Keyword
- fluorescence
- Subject Keyword
- biomedical
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Physics and Astronomy
- Identifier
- http://hdl.handle.net/1959.14/130818
- Identifier
- ISSN:1572-896X
- Identifier
- mq-rm-2010005234
- Language
- eng
- Reviewed
