Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/126164
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- Title
- Quantum-dot-functionalized poly(styrene-co-acrylic acid) microbeads : step-wise self-assembly, characterization, and applications for sub-femtomolar electrochemical detection of DNA hybridization
- Related
- Advanced functional materials, Vol. 20, No. 7 (2010), p.1173-1179
- DOI
- 10.1002/adfm.200901721
- Publisher
- Wiley-VCH Verlag GmbH
- Date
- 2010
- FoR/RFCD Code(s)
-
090400 Chemical Engineering
030300 Macromolecular and Materials Chemistry
091200 Materials Engineering
- Author/Creator
- Dong, Haifeng
- Author/Creator
- Yan, Feng
- Author/Creator
- Ji, Hanxu
- Author/Creator
- Wong, Danny K. Y
- Author/Creator
- Ju, Huangxian
- Description
- A novel nanoparticle label capable of amplifying the electrochemical signal of DNA hybridization is fabricated by functionalizing poly(styrene-co-acrylic acid) microbeads with CdTe quantum dots. CdTe-tagged polybeads are prepared by a layer-by-layer self-assembly of the CdTe quantum dots (diameter = 3.07 nm) and polyelectrolyte on the polybeads (diameter = 323 nm). The self-assembly procedure is characterized using scanning and transmission electron microscopy, and X-ray photoelectron, infrared and photoluminescence spectroscopy. The mean quantum-dot coverage is (9.54 ± 1.2) × 10³ per polybead. The enormous coverage and the unique properties of the quantum dots make the polybeads an effective candidate as a functionalized amplification platform for labelling of DNA or protein. Herein, as an example, the CdTe-tagged polybeads are attached to DNA probes specific to breast cancer by streptavidin–biotin binding to construct a DNA biosensor. The detection of the DNA hybridization process is achieved by the square-wave voltammetry of Cd²⁺ after the dissolution of the CdTe tags with HNO₃. The efficient carrier-bead amplification platform, coupled with the highly sensitive stripping voltammetric measurement, gives rise to a detection limit of 0.52 fmol L⁻¹ and a dynamic range spanning 5 orders of magnitude. This proposed nanoparticle label is promising, exhibits an efficient amplification performance, and opens new opportunities for ultrasensitive detection of other biorecognition events.
- Description
- 7 page(s)
- Subject Keyword
- 090400 Chemical Engineering
- Subject Keyword
- 030300 Macromolecular and Materials Chemistry
- Subject Keyword
- 091200 Materials Engineering
- Subject Keyword
- DNA biosensors
- Subject Keyword
- layer-by-layer assembly
- Subject Keyword
- polybeads
- Subject Keyword
- quantum dots
- Subject Keyword
- signal amplification
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/126164
- Identifier
- ISSN:1616-301X
- Identifier
- mq-rm-2010004951
- Language
- eng
- Reviewed
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