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Evaluation of blood collection tubes using selected reaction monitoring MS : implications for proteomic biomarker studies

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/126012

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Title: Evaluation of blood collection tubes using selected reaction monitoring MS : implications for proteomic biomarker studies
Related: Proteomics, Vol. 10, No. 10 (2010), p.2050-2056
DOI: 10.1002/pmic.200900517
Publisher: Wiley-VCH Verlag GmbH
Date: 2010
FoR/RFCD Code(s): 060100 Biochemistry and Cell Biology
Author/Creator: Randall, Sarah A
Author/Creator: McKay, Matthew J
Author/Creator: Molloy, Mark P
Description: An emphasis of current proteomic research is the validation of plasma protein biomarkers. The process of blood collection itself is critical to the accuracy and reproducibility of quantitative biomarker assays. We have developed selected reaction monitoring (SRM) assays to analyse thirteen abundant plasma proteins and evaluated the impact of three different blood collection tubes on the levels of these proteins. We also assessed the implications of the time taken to analyse plasma samples by evaluating the recovery of these proteins. We showed that SRM detects minor differences in the levels of some proteins which can be attributed to collection tube type. The average recovery for 12 of 18 assays was higher for proteins that were collected in tubes containing protease inhibitors compared to conventional collection tubes. For five of the assays, the differential recovery was statistically significant. Delaying MS analysis of a freeze-thawed sample for 1 hour showed greatly reduced recovery of these analytes; however differences attributed to tube type were only evident at the baseline timepoint. Finally, we assessed the natural variation of circulating levels of these proteins in a cohort of seven healthy individuals. This study provides useful information for researchers contemplating blood collection for undertaking protein biomarker studies.
Description: 7 page(s)
Subject Keyword: 060100 Biochemistry and Cell Biology
Subject Keyword: biomarker
Subject Keyword: biomedicine mass spectrometry
Subject Keyword: plasma
Subject Keyword: quantitation
Subject Keyword: selected reaction monitoring
Resource Type: journal article
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/126012
Identifier: ISSN:1615-9853
Identifier: mq-rm-2010004959
Language: eng
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