Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/125574
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- Title
- Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles
- Related
- Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues (8th : 2010) (23 - 25 January 2010 : San Francisco, California)
- Related
- Farkas, Daniel L.; Nicolau, Dan V. and Leif, Robert C.. Imaging, manipulation, and analysis of biomolecules, cells, and tissues VIII : 23-25 January 2010, San Francisco, California, United States, p.756819-1-756819-12
- DOI
- 10.1117/12.841569
- Related
- Proceedings of SPIE Vol. 7568
- Publisher
- Bellingham, Washington : SPIE
- Date
- 2010
- FoR/RFCD Code(s)
-
030500 Organic Chemistry
060500 Microbiology
- Author/Creator
- Jin, Dayong
- Author/Creator
- Piper, James
- Author/Creator
- Yuan, Jingli
- Author/Creator
- Leif, Robert
- Description
- In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.
- Description
- 12 page(s)
- Subject Keyword
- 030500 Organic Chemistry
- Subject Keyword
- 060500 Microbiology
- Subject Keyword
- time-gated luminescence
- Subject Keyword
- real-time imaging
- Subject Keyword
- autofluorescence
- Subject Keyword
- silica nanoparticle
- Subject Keyword
- europium
- Subject Keyword
- terbium
- Subject Keyword
- lanthanide
- Subject Keyword
- waterborne
- Subject Keyword
- pathogen
- Subject Keyword
- microscopy
- Subject Keyword
- lifetime
- Resource Type
- conference paper
- Organisation
- Macquarie University. Dept. of Physics and Astronomy
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/125574
- Identifier
- ISBN:9780819479648
- Identifier
- ISSN:0277-786X
- Identifier
- mq-rm-2009011288
- Language
- eng
- Full Text
- Reviewed
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