Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues (8th : 2010) (23 - 25 January 2010 : San Francisco, California)
Farkas, Daniel L.; Nicolau, Dan V. and Leif, Robert C.. Imaging, manipulation, and analysis of biomolecules, cells, and tissues VIII : 23-25 January 2010, San Francisco, California, United States, p.756819-1-756819-12
In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.