Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/124962
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- Title
- Determining blood cell size using microfluidic hydrodynamics
- Related
- Journal of immunological methods, Vol. 329, Issue 1-2 (2008), p.151-156
- DOI
- 10.1016/j.jim.2007.10.004
- Publisher
- Elsevier BV
- Date
- 2008
- FoR/RFCD Code(s)
-
110700 Immunology
111300 Ophthalmology and Optometry
- Author/Creator
- Inglis, David W
- Author/Creator
- Davis, John A
- Author/Creator
- Zieziulewicz, Thomas J
- Author/Creator
- Lawrence, David A
- Author/Creator
- Austin, Robert H
- Author/Creator
- Sturm, James C
- Description
- Microfluidic flow cytometers currently analyze far fewer parameters than conventional flow cytometry or fluorescence activated cell sorting (FACS) in order to minimize cost and complexity. There is a need for microfluidic devices that analyze more and or new cell parameters with compact and minimal means. Here we show a new and explicitly microfluidic parameter, “hydrodynamic” cell size, and compare it to forward scatter in conventional flow cytometry. The hydrodynamic size of cells is determined by the degree of lateral displacement experienced while traveling through a 1.2-mm-wide non-clogging array of micro-fabricated obstacles. We show comparable size resolution between the microfluidic device and forward scatter in conventional flow cytometry and without the need to lyse red blood cells. We use the device to differentiate healthy lymphocytes from malignant lymphocytes by size alone and we use the device to detect increased numbers of activated lymphocytes in blood as a result of exposure to staphylococcal enterotoxin B (SEB), a potential bioterror agent. Together the results demonstrate a microfluidic device that performs some of the measurement and separation tasks of a flow cytometer but at a potentially lower cost and complexity.
- Description
- 6 page(s)
- Subject Keyword
- 110700 Immunology
- Subject Keyword
- 111300 Ophthalmology and Optometry
- Subject Keyword
- microfluidic
- Subject Keyword
- flow cytometry
- Subject Keyword
- separation
- Subject Keyword
- SEB
- Subject Keyword
- J45
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Physics and Astronomy
- Identifier
- http://hdl.handle.net/1959.14/124962
- Identifier
- ISSN:0022-1759
- Identifier
- mq-rm-2008002396
- Language
- eng
- Reviewed
