Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/124441
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- Title
- Preparation and time-resolved luminescence bioassay application of multicolor luminescent lanthanide nanoparticles
- Related
- Journal of fluorescence, Vol. 20, No. 1 (2009), p.321-328
- DOI
- 10.1007/s10895-009-0559-7
- Publisher
- Springer
- Date
- 2009
- FoR/RFCD Code(s)
-
030600 Physical Chemistry (incl. Structural
030100 Analytical Chemistry
- Author/Creator
- Jiang, Hongfei
- Author/Creator
- Wang, Guilan
- Author/Creator
- Zhang, Wenzhu
- Author/Creator
- Liu, Xiaoyu
- Author/Creator
- Ye, Zhiqiang
- Author/Creator
- Jin, Dayong
- Author/Creator
- Yuan, Jingli
- Author/Creator
- Liu, Zhiguang
- Description
- Because highly luminescent lanthanide compounds are limited to Eu(3+) and Tb(3+) compounds with red (Eu, approximately 615 nm) and green (Tb, approximately 545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu(3+)-Tb(3+) complexes with a ligand N,N,N(1),N(1)-(4'-phenyl-2,2':6',2''-terpyridine-6,6''-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.
- Description
- 8 page(s)
- Subject Keyword
- 030600 Physical Chemistry (incl. Structural
- Subject Keyword
- 030100 Analytical Chemistry
- Subject Keyword
- lanthanide
- Subject Keyword
- multicolor luminescent nanoparticle
- Subject Keyword
- biolabeling
- Subject Keyword
- time-resolved luminescence bioassay
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Physics and Engineering
- Identifier
- http://hdl.handle.net/1959.14/124441
- Identifier
- ISSN:1053-0509
- Identifier
- mq-rm-2009005922
- Language
- eng
- Reviewed
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