Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/123870
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- Title
- UV LED excited time-gated luminescence flow cytometry : concepts and experimental evaluation
- Related
- Smart Medical and Biomedical Sensor Technology (4th : 2006) (3 - 4 October 2006 : Boston, Massachusetts)
- Related
- Cullum, Brian M. and Carter, J. Chance. Smart medical and biomedical sensor technology IV : 3-4 October 2006, Boston, Massachusetts, USA, p.63800L-1-63800L-8
- DOI
- 10.1117/12.685076
- Related
- Proceedings of SPIE Vol. 6380
- Publisher
- Bellingham, Washington : SPIE
- Date
- 2006
- FoR/RFCD Code(s)
-
020500 Optical Physics
090600 Electrical and Electronic Engineering
100200 Environmental Biotechnology
- Author/Creator
- Jin, Dayong
- Author/Creator
- Connally, Russell
- Author/Creator
- Piper, James A
- Description
- This paper presents experimental and theoretical studies of time-gated discrimination of long-lived luminescence (lifetime: 1~2000μs) labelled target-organisms against non-target autofluorescence background (lifetime: <100 ns) in flow cytometry. A theoretical model of such a TGL flow cytometer is developed which takes account of flow speed, illumination and detection apertures, fluorescence label lifetime, and pulsed illumination and gated detection timing sequences. Ultraviolet LED and channel photomultiplier were found to be practical as pulsed excitation sources and gated detector for TGL flow cytometry. The prototype cytometer was constructed and optimized to operate at 6 k Hz repetition rate of TGL cycles consisting of 100 μs LED excitation and ~60 μs gated detection. The spatial counting efficiency was evaluated by enumerating 5.5 μm diameter europium microspheres resulting in a counting accuracy approaching 100%.
- Description
- 8 page(s)
- Subject Keyword
- 020500 Optical Physics
- Subject Keyword
- 090600 Electrical and Electronic Engineering
- Subject Keyword
- 100200 Environmental Biotechnology
- Subject Keyword
- flow cytometry
- Subject Keyword
- ultraviolet light emitting diode (UV LED)
- Subject Keyword
- time-gated luminescence (TGL)
- Subject Keyword
- channel photomultiplier tube (CPM)
- Subject Keyword
- lanthanide chelate
- Subject Keyword
- rare-event
- Subject Keyword
- microbial
- Resource Type
- conference paper
- Organisation
- Macquarie University. Dept. of Physics
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/123870
- Identifier
- ISBN:9780819464781
- Identifier
- ISSN:0277-786X
- Identifier
- mq-rm-2008001996
- Language
- eng
- Reviewed
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