Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/120199
27 Visitors
29 Hits
1 Downloads
- Title
- Ultrasensitive time-resolved nanoliter volume fluorometry based on UV LEDs and a channel photomultiplier tube
- Related
- Imaging, Manipulation, and Analysis of Biomolecules and Cells : Fundamentals and Applications (3rd : 2005) (24 - 27 March 2005 : San Jose, California)
- Related
- Nicolau; Dan V.; Enderlein, Joerg; Leif, Robert C.; Farkas, Daniel L. and Raghavachari, Ramesh. Imaging, manipulation, and analysis of biomolecules and cells : fundamentals and applications III : 24-27 January 2005, San Jose, California, USA, p.237-245
- DOI
- 10.1117/12.590127
- Related
- Proceedings of SPIE Vol. 5699
- Publisher
- Bellingham, Washington : SPIE
- Date
- 2005
- FoR/RFCD Code(s)
-
020500 Optical Physics
090600 Electrical and Electronic Engineering
100200 Environmental Biotechnology
- Author/Creator
- Jin, Dayong
- Author/Creator
- Connally, Russell
- Author/Creator
- Piper, Jim
- Description
- A capillary fluorometer was constructed using a 2 mW, 365 nm ultraviolet (UV) light emitting diode (LED) as the excitation source and a new-generation high-gain (3×10⁸) channel photomultiplier tube. The use of a LED permitted rapid pulsing of the excitation source so that the instrument could be employed for time-resolved fluorescence (TRF) applications. A detection limit of ~2×10⁸ molecules of BHHT (4,4’-bis (1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"yl)-o-tephenyl)-Eu (III) were resolved within a 1.25 nanoliter volume at a S/N ratio of 3:1. Ultimate sensitivity of the system was compromised due to visible luminescence emitted by the UV LED, centred around 550 nm extending to > 700 nm and 2nd-order exponentially decaying with lifetimes of 40 μs and 490 μs.
- Description
- 9 page(s)
- Subject Keyword
- 020500 Optical Physics
- Subject Keyword
- 090600 Electrical and Electronic Engineering
- Subject Keyword
- 100200 Environmental Biotechnology
- Subject Keyword
- ultraviolet light emitting diodes (UV LEDs)
- Subject Keyword
- time-resolved fluorescence (TRF)
- Subject Keyword
- channel photomultiplier tube (CPM)
- Subject Keyword
- lanthanide chelate
- Subject Keyword
- BHHT
- Resource Type
- conference paper
- Organisation
- Macquarie University. Dept. of Physics
- Organisation
- Macquarie University. Office of the Deputy Vice-Chancellor (Research)
- Identifier
- http://hdl.handle.net/1959.14/120199
- Identifier
- ISBN:081945673X
- Identifier
- ISSN:0277-786X
- Identifier
- mq-rm-2008001991
- Language
- eng
- Full Text
- Reviewed
-
-
List Of Titles
