Europium chelate (BHHCT-Eu³⁺) and its metal nanostructure enhanced luminescence applied to bioassays and time-gated bioimaging | Macquarie University ResearchOnline

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Europium chelate (BHHCT-Eu³⁺) and its metal nanostructure enhanced luminescence applied to bioassays and time-gated bioimaging

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/119873

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Title: Europium chelate (BHHCT-Eu³⁺) and its metal nanostructure enhanced luminescence applied to bioassays and time-gated bioimaging
Related: Langmuir : the ACS journal of surfaces and colloids, Vol. 26, No. 12 (2010), p.10036-10043
DOI: 10.1021/la100158g
Publisher: American Chemical Society
Date: 2010
FoR/RFCD Code(s): 030600 Physical Chemistry (incl. Structural 090400 Chemical Engineering 090300 Biomedical Engineering
Author/Creator: Deng, Wei
Author/Creator: Jin, Dayong
Author/Creator: Drozdowicz-Tomsia, Krystyna
Author/Creator: Yuan, Jingli
Author/Creator: Goldys, Ewa M
Description: We report the use of europium chelate, 4,4′-bis(1′′,1′′,1′′,2′′,2′′,3′′,3′′-heptafluoro-4′′,6′′-hexanedion-6′′-yl)chlorosulfo-o-terphenyl-Eu³⁺(BHHCT-Eu³⁺), in silver nanostructure-enhanced luminescence and its application to bioassays and bioimaging. The highest luminescence intensity enhancement factor of BHHCT-Eu³⁺ achieved in this study was about 11 times, while the simultaneously measured luminescence lifetime was reduced 2-fold. The luminophore photostability was also improved by a factor of 3. On the basis of these experimental results, we estimated the impact of silver nanostructures on the excitation and emission enhancement factors. Luminescence enhancement was demonstrated in two geometries: on planar glass substrates and on silica beads. In the biotin-modified IgG antibody assay the bead geometry provided slightly higher enhancement factor and greater sensitivity. Subsequently, we applied such bead substrates to time-gated luminescence imaging of Giardia lamblia cells stained by BHHCT-Eu3+ where we observed improved brightness by a factor of 2. Such improved photostability and brightness of BHHCT-Eu³⁺ in the presence of metal nanostructures are highly desirable for ultrasensitive bioassays and bioimaging, especially with time gating.
Description: 8 page(s)
Subject Keyword: 030600 Physical Chemistry (incl. Structural
Subject Keyword: 090400 Chemical Engineering
Subject Keyword: 090300 Biomedical Engineering
Resource Type: journal article
Organisation: Macquarie University. Dept. of Physics and Astronomy
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/119873
Identifier: ISSN:1520-5827
Identifier: mq-rm-2010004603
Language: eng
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