In this study we examined the effects of varying stimulus check size on multifocal visual evoked potential (VEP). We also evaluated the currently used cortical scaling of stimulus segments. The ObjectiVisionTM multifocal objective perimeter stimulates the eye with random check patterns at 56 cortically scaled segments within the visual field extending to a radius of 26°. All cortically scaled segments have equal number of checks, which gradually increase in size from the center to the periphery, proportional to the size of the segment. Stimuli with 9, 16, 25, 36 and 49 checks/segment were tested on 10 eyes belonging to 10 normal subjects. The check size varied inversely with number of checks per segment. VEP was recorded using bipolar occipital cross electrodes (7min/eye), the amplitude and latency of responses obtained were compared with the check size at different eccentricities. Our findings suggest that the existing setting with 16 checks/segment subtending 26′ to 140′ from center to periphery, is the most effective amongst all the check sizes. Decreasing the check size prolongs the latency in the central field only. Cortical scaling of segments generates responses of the same order of magnitude throughout the field, but could be improved slightly to enhance the signal from the outer two rings.