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Development of resistance to insulin-like growth factor binding protein-3 in transfected T47D breast cancer cells

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/115384

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Title: Development of resistance to insulin-like growth factor binding protein-3 in transfected T47D breast cancer cells
Related: Biochemical and biophysical research communications, Vol. 246, Issue 2 (1998), p.325-329
DOI: 10.1006/bbrc.1998.8615
Publisher: Academic Press
Date: 1998
Author/Creator: Firth, Sue M
Author/Creator: Fanayan, Susan
Author/Creator: Benn, Diana
Author/Creator: Baxter, Robert C
Description: Insulin-like growth factor binding protein-3 (IGFBP-3) has antiproliferative effects in many cell types but paradoxical growth stimulation has also been reported. In early passages following transfection of T47D breast cancer cells with IGFBP-3 cDNA, the proliferation rate and serum-stimulated DNA synthesis were significantly reduced compared to control cells. Cell cycle analysis indicated that growth-inhibited IGFBP-3-producing cells accumulated in G1phase. After several passages, the transfected cells became resistant to the inhibitory effects of IGFBP-3 and showed transiently enhanced proliferation rates despite an increased IGFBP-3 concentration in the medium. IGFBP-3 proteolysis did not account for its decreased antiproliferative activity in resistant cells. We hypothesize that development of resistance to the antiproliferative action of IGFBP-3 might be an important step in the malignant progression of breast cancer cells.
Description: 5 page(s)
Resource Type: journal article
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/115384
Identifier: ISSN:0006-291X
Identifier: mq-rm-2010002641
Language: eng
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