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- Title
- Bioinformatic analysis of abundant, gender-enriched transcripts of adult Ascaris suum (Nematoda) using a semi-automated workflow platform
- Related
- Molecular and cellular probes, Vol. 23, No. 5 (2009), p.205-217
- DOI
- 10.1016/j.mcp.2009.03.003
- Publisher
- Elsevier
- Date
- 2009
- Author/Creator
- Cantacessi, C
- Author/Creator
- Zou, F. C
- Author/Creator
- Hall, R. S
- Author/Creator
- Zhong, W
- Author/Creator
- Campbell, B. E
- Author/Creator
- Ranganathan, S
- Author/Creator
- Sternberg, P. W
- Author/Creator
- Zhu, X. Q
- Author/Creator
- Gasser, R. B
- Description
- Expressed sequence tag (EST) data representing transcripts with a high level of differential hybridization in suppressive-subtractive hybridization (SSH)-based microarray analysis between adult female and male Ascaris suum were subjected to detailed bioinformatic analysis. A total of 361 ESTs clustered into 209 sequences, of which 52 and 157 represented transcripts that were enriched in female and male A. suum, respectively. Thirty (57.7%) of the ‘female’ subset of 52 sequences had orthologues/homologues in other parasitic nematodes and/or Caenorhabditis elegans, 13 (25%) exclusively in other parasitic nematodes and nine (17.3%) had no match in any other organism for which sequence data are currently available; the C. elegans orthologues encoded molecules involved in reproduction as well as embryonic and gamete development, such as vitellogenins and chitin-binding proteins. Of the ‘male’ subset of 157 sequences, 73 (46.5%) had orthologues/homologues in other parasitic nematodes and/or C. elegans, 57 (37.5%) in other parasitic nematodes only, and 22 (14.5%) had no significant similarity match in any other organism; the C. elegans orthologues encoded predominantly major sperm proteins (MSPs), kinases and phosphatases, actins, myosins and an Ancylostoma secreted protein-like molecule. The findings of the present study should support further genomic investigations of A. suum.
- Description
- 13 page(s)
- Subject Keyword
- 030400 Medicinal and Biomolecular Chemistry
- Subject Keyword
- 060100 Biochemistry and Cell Biology
- Subject Keyword
- Ascaris suum
- Subject Keyword
- gender-enriched
- Subject Keyword
- suppressive-subtractive hybridization
- Subject Keyword
- Caenorhabditis elegans
- Subject Keyword
- bioinformatics
- Subject Keyword
- gene ontology
- Subject Keyword
- genetic interactions
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/114154
- Identifier
- ISSN:0890-8508
- Identifier
- mq-rm-2009002826
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
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