Add to Quick Collection
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/111484
239 Visitors
205 Hits
0 Downloads
- Title
- ¹⁸O-Labelling of chlorophyll d in Acaryochloris marina reveal chlorophyll a and molecular oxygen are precursors
- Related
- Journal of biological chemistry, Vol. 285, No. 37 (2010), p.28450-28456
- DOI
- 10.1074/jbc.M110.146753
- Publisher
- American Society for Biochemistry and Molecular Biology
- Date
- 2010
- Author/Creator
- Schliep, Martin
- Author/Creator
- Crossett, Ben
- Author/Creator
- Willows, Robert D
- Author/Creator
- Chen, Min
- Description
- The cyanobacterium Acaryochloris marina was cultured in the presence of either H2¹⁸O or ¹⁸O2, and the newly synthesized chlorophylls (Chl a and Chl d) were isolated using high performance liquid chromatography and analyzed by mass spectroscopy. In the presence of H2¹⁸O, newly synthesized Chl a and d, both incorporated up to four isotopic ¹⁸O atoms. Time course H2¹⁸O labeling experiments showed incorporation of isotopic ¹⁸O atoms originating from H2¹⁸O into Chl a, with over 90% of Chl a ¹⁸O-labeled at 48 h. The incorporation of isotopic ¹⁸O atoms into Chl d upon incubation in H2¹⁸O was slower compared with Chl a with ∼50% ¹⁸O-labeled Chl d at 115 h. The rapid turnover of newly synthesized Chl a suggested that Chl a is the direct biosynthetic precursor of Chl d. In the presence of ¹⁸O2 gas, one isotopic ¹⁸O atom was incorporated into Chl a with approximately the same kinetic incorporation rate observed in the H2¹⁸O labeling experiment, reaching over 90% labeling intensity at 48 h. The incorporation of two isotopic ¹⁸O atoms derived from molecular oxygen (¹⁸O2) was observed in the extracted Chl d, and the percentage of double isotopic ¹⁸O-labeled Chl d increased in parallel with the decrease of non-isotopic-labeled Chl d. This clearly indicated that the oxygen atom in the C3¹-formyl group of Chl d is derived from dioxygen via an oxygenase-type reaction mechanism.
- Description
- 7 page(s)
- Subject Keyword
- 060100 Biochemistry and Cell Biology
- Subject Keyword
- chlorophyll d
- Subject Keyword
- photosynthesis
- Subject Keyword
- biosynthesis
- Subject Keyword
- enzyme
- Subject Keyword
- mechanism
- Resource Type
- journal article
- Organisation
- Macquarie University. Department of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/111484
- Identifier
- mq:11861
- Identifier
- ISSN:0021-9258
- Identifier
- mq-rm-2009011259
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
