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Use of graphitised carbon negative ion LC-MS to analyse enzymatically digested glycosaminoglycans

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/103931

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Title: Use of graphitised carbon negative ion LC-MS to analyse enzymatically digested glycosaminoglycans
Related: Microbiology, Vol. 824, Issue 1-2 (2005), p.139-147
DOI: 10.1016/j.jchromb.2005.07.014
Publisher: Elsevier
Date: 2005
FoR/RFCD Code(s): 030100 Analytical Chemistry
Author/Creator: Karlsson, Niclas G
Author/Creator: Schulz, Benjamin L
Author/Creator: Packer, Nicolle H
Author/Creator: Whitelock, John M
Description: Capillary liquid chromatography–mass spectrometry using graphitised carbon stationary phase and ion trap mass spectrometry was shown to be a powerful technique for analysing glycosaminoglycans digested with endoglycosidases. Commonly found disaccharides from heparin/heparan sulphate digests at sub nanomole levels were found to be separated by mass and/or retention time and detected by negative ion electrospray mass spectrometry predominantly as [M − H]− ions using a standard electrospray interface and flow rate between 6–10 μL/min. Graphitised carbon liquid chromatography–fragmentation mass spectrometry provided sequence data of disaccharides and oligosaccharides. Sequence information was obtained from either collision of the [M − H]− ions (low sulphated disaccharides) or of the [M + Na − 2H]− ions (highly sulphated disaccharides). This separation and identification method of endoglycosidase digestion and sample preparation using a combination of cation exchange and graphitised carbon, was used to successfully analyse digests of keratan sulphate (keratanase) and heparin (heparinase) standards, and hyaluronic acid (hyaluronidase) from synovial fluid samples.
Description: 9 page(s)
Subject Keyword: 030100 Analytical Chemistry
Subject Keyword: proteoglycans
Subject Keyword: hyaluronic acid
Subject Keyword: keratan sulphate
Subject Keyword: heparin
Subject Keyword: heparan sulphate
Resource Type: journal article
Organisation: Macquarie University. Dept. of Chemistry and Biomolecular Sciences

Identifier: http://hdl.handle.net/1959.14/103931
Identifier: ISSN:1570-0232
Identifier: mq-rm-2006010853
Language: eng
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