Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/103783
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- Title
- Glycoproteomics of milk : differences in sugar epitopes on human and Bovine milk fat globule membranes
- Related
- Journal of proteome research, Vol. 7, No. 9 (2008), p.3687-3696
- DOI
- 10.1021/pr700793k
- Publisher
- American Chemical Society
- Date
- 2008
- FoR/RFCD Code(s)
-
060100 Biochemistry and Cell Biology
- Author/Creator
- Wilson, Nicole H
- Author/Creator
- Robinson, Leanne J
- Author/Creator
- Donnet, Anne
- Author/Creator
- Bovetto, Lionel
- Author/Creator
- Packer, Nicolle H
- Author/Creator
- Karlsson, Niclas G
- Description
- Oligosaccharides from human and bovine milk fat globule membranes were analyzed by LC-MS and LC-MS/MS. Global release of N-linked and O-linked oligosaccharides showed both to be highly sialylated, with bovine peak-lactating milk O-linked oligosaccharides presenting as mono- and disialylated core 1 oligosaccharides (Galβ1-3GalNAcol), while human milk had core type 2 oligosaccharides (Galβ1-3(GlcNAcβ1-6)GalNAcol) with sialylation on the C-3 branch. The C-6 branch of these structures was extended with branched and unbranched N-acetyllactosamine units terminating in blood group H and Lewis type epitopes. These epitopes were also presented on the reducing terminus of the human, but not the bovine, N-linked oligosaccharides. The O-linked structures were found to be attached to the high molecular mass mucins isolated by agarose-polyacrylamide composite gel lectrophoresis, where MUC1 and MUC4 were present. Analysis of bovine colostrum showed that O-linked core 2 oligosaccharides are present at the early stage (3 days after birth) but are down-regulated as lactation develops. This data indicates that human milk may provide different innate immune protection against pathogens compared to bovine milk, as evidenced by the presence of Lewis b epitope, a target for the Helicobacter pylori bacteria, on human, but not bovine, milk fat globule membrane mucins. In addition, non-mucintype O-linked fucosylated oligosaccharides were found (NeuAc-Gal-GlcNAc1-3Fuc-ol in bovine milk and Gal-GlcNAc1-3Fuc-ol in human milk). The O-linked fucose structure in human milk is the first to our knowledge to be found on high molecular mass mucin-type molecules.
- Description
- 10 page(s)
- Subject Keyword
- 060100 Biochemistry and Cell Biology
- Subject Keyword
- milk
- Subject Keyword
- MUC1
- Subject Keyword
- MUC4
- Subject Keyword
- mucin
- Subject Keyword
- EGF
- Subject Keyword
- bacterial adhesion
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/103783
- Identifier
- ISSN:1535-3893
- Identifier
- mq-rm-2007011940
- Language
- eng
- Reviewed
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